Fixtures are subject to change due to postponements and cup matches.
| Date | Team | Location | Type | Result | Scorers |
|---|---|---|---|---|---|
| 16.01.2021 | Barnsley | Away | League | ||
| 23.01.2021 | Calderdale | Home | League | ||
| 30.01.2021 | Doncaster | Away | League | ||
| 06.02.2021 | East Riding | Home | League | or ESFA Rd 1 | |
| 13.02.2021 | Harrogate | Away | League | ||
| 20.02.2021 | Rotherham | Home | League | ||
| 27.02.2021 | Wakefield | Away | League | ||
| 06.03.2021 | ESFA Rd 2 | ||||
| 13.03.2021 | Spen Valley | Away | League | ||
| 20.03.2021 | Sheffield | Home | League | ||
| 27.03.2021 | Barnsley | Home | League | or ESFA Rd 3 | |
| 03.04.2021 | Calderdale | Away | League | Easter | |
| 10.04.2021 | Doncaster | Home | League | ||
| 17.04.2021 | East Riding | Away | League | ||
| 24.04.2021 | Harrogate | Home | League | ||
| 01.05.2021 | Rotherham | Away | League | or ESFA Rd 4 | |
| 08.05.2021 | Wakefield | Home | League | ||
| 15.05.2021 | Spen Valley | Home | League | ||
| 22.05.2021 | Sheffield | Away | League | or ESFA Rd 5 | |
| 29.05.2021 | |||||
| 05.06.2021 | |||||
| 12.06.2021 | ESFA Rd 6 |